diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm
index d9e5e5a54e..d25dc8d193 100644
--- a/gnu/packages/bioinformatics.scm
+++ b/gnu/packages/bioinformatics.scm
@@ -981,14 +981,6 @@ (define (cythonized? c/c++-file)
 Python.")
     (license license:gpl2+)))
 
-(define-public python2-pybedtools
-  (let ((pybedtools (package-with-python2 python-pybedtools)))
-    (package
-      (inherit pybedtools)
-      (native-inputs
-       (modify-inputs (package-native-inputs pybedtools)
-         (prepend python2-pathlib))))))
-
 (define-public python-biom-format
   (package
     (name "python-biom-format")